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nevoltris d antibody engineering methods protocols 3ed 2018

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Textbook in PDF format This detailed new edition provides complete and easy access to a variety of antibody engineering techniques. The volume explores topics such as the generation of native, synthetic, or immune antibody libraries, the selection of lead candidates via the different powerful and innovative display technologies, Fc engineering, as well as their production, characterization, and optimization of antibodies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Antibody Engineering: Methods and Protocols, Third Edition presents the reader with an extensive toolbox to create the powerful molecules of tomorrow. In Silico Methods Antibody Design and Humanization via In Silico Modeling Antibody Affinity Maturation by Computational Design Use of IMGT Databases and Tools for Antibody Engineering and Humanization Generation of Diversity Construction of Human Naïve Antibody Gene Libraries Construction of Synthetic Antibody Libraries Construction of Histidine-Enriched Shark IgNAR Variable Domain Antibody Libraries for the Isolation of pH-Sensitive vNAR Fragments Display Technologies for Generation of Ig Single Variable Domains A Streamlined Approach for the Construction of Large Yeast Surface Display Fab Antibody Libraries Selections of Lead Candidate Phage Display and Selections on Purified Antigens Selection of Antibodies to Transiently Expressed Membrane Proteins Using Phage Display Selection of Antibody Fragments Against Structured DNA by Phage Display Selection of Antibody Fragments by Yeast Display Rapid Selection of High-Affinity Antibody scFv Fragments Using Ribosome Display In Vitro Selection of Single-Domain Antibody (VHH) Using cDNA Display Sequencing and Affinity Determination of Antigen-Specific B Lymphocytes from Peripheral Blood Production of Recombinant Antibodies Expression of IgG Monoclonals with Engineered Immune Effector Functions An IRES-Mediated Tricistronic Vector for Efficient Generation of Stable, High-Level Monoclonal Antibody Producing CHO DG44 Cell Lines Production, Purification, and Characterization of Antibody-TNF Superfamily Ligand Fusion Proteins Fc Engineering Chemoenzymatic Defucosylation of Therapeutic Antibodies for Enhanced Effector Functions Using Bacterial α-Fucosidases Fc Glyco- and Fc Protein-Engineering: Design of Antibody Variants with Improved ADCC and CDC Activity Fc Engineering: Tailored Synthetic Human IgG1-Fc Repertoire for High-Affinity Interaction with FcRn at pH 6.0 Characterization, Optimization, and Innovative Formats Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance Parallel Evolution of Antibody Affinity and Thermal Stability for Optimal Biotherapeutic Development The Use of Somatic Hypermutation for the Affinity Maturation of Therapeutic Antibodies Selection and Use of Intracellular Antibodies Site-Specific Radioactive Labeling of Nanobodies

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nevoltris d antibody engineering methods protocols 3ed 2018

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