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bhat characterization plant viruses methods protocols 2020

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Textbook in PDF format This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses on in vitro expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its scope, the book will be a valuable resource for every laboratory, student and teacher, and for everyone interested in plant virology, plant pathology, plant biology and molecular biology, offering them a practical manual on various aspects of plant viruses. Foreword Preface About the Book About the Authors Abbreviations Glasshouse for Maintenance of Virus and Insect Culture Symptoms of Virus-Infected Plants Isolation and Diagnosis of Virus Through Indicator Hosts Host Range of Viruses Physico-chemical Properties of Virus in Crude Sap Mechanical Sap Transmission Transmission Through Grafting and Budding Transmission Through Dodder Virus Transmission Through Pollen Transmission Through Seeds Transmission of Viruses by Aphids Transmission of Viruses by Leafhoppers Transmission of Viruses by Whiteflies Transmission of Viruses by Thrips Transmission of Viruses Through Mealybugs Transmission of Viruses Through Beetles Transmission of Viruses Through Mites Transmission of Viruses Through Fungi Transmission of Viruses Through Nematodes Storage and Preservation of Plant Virus Cultures Purification of Plant Viruses Ultraviolet Absorption Spectra of Purified Virus Preparation Electron Microscopy and Utramicrotomy Determination of Coat Protein Molecular Weight of Viruses Isolation of Nucleic Acid from Purified Virus and Determination of Its Nature Agarose Gel Electrophoresis for Nucleic Acids In Vitro Expression of Viral Coat Protein in Prokaryotic System and Its Purification Production of Polyclonal Antiserum Production of Monoclonal Antibody Serological Tests Isolation of Total DNA from Plants Isolation of Total RNA from Plants Isolation of Double-Stranded (ds) RNA from Virus-Infected Plants Dot-Blot Hybridization Technique Polymerase Chain Reaction Real-Time Polymerase Chain Reaction DNA Microarray for Detection of Plant Viruses Loop-Mediated Isothermal Amplification (LAMP) Rolling Circle Amplification (RCA) Recombinase Polymerase Amplification Next-Generation Sequencing for Diagnosis of Viruses Cloning of PCR Product cDNA Synthesis and Cloning DNA Sequencing Sequence Analysis and Phylogenetic Studies Development of Infectious Clone of Virus Virus Elimination by Meristem-Tip Culture Virus Elimination Through Somatic Embryogenesis Production of Virus-Resistant Plants Through Transgenic Approaches Production of Virus-Resistant Plants Through CRISPR-Cas Technology Appendix: Common Conversions, Information Sources and Software of Nucleic Acids and ProteinsWeight conversion1 μg = 10-6 g1 ng... DNA Data Important DNA and Protein Information Sources and Software Software for Sequence Analysis Glossary

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bhat characterization plant viruses methods protocols 2020