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parrish n pirna methods protocols 2022

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Textbook in PDF format This detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several bioinformatic and biophysical methods related to piRNA studies, consistent with the increasing importance of high-throughput sequencing and computational methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, piRNA: Methods and Protocols serves as an ideal guide for researchers seeking to elucidate the numerous mysteries of this area of multicellular biology. Methods to Study the Nature and Function of piRNAs in Non-classical Organisms Functional Analysis of Individual piRNAs in Aedes aegypti Cells and Embryos Using Antisense Oligonucleotides CRISPR-Mediated Genome Engineering in Aedes aegypti PIWI-Directed DNA Elimination for Tetrahymena Genetics Planarian PIWI–piRNA Interaction Analysis Using Immunoprecipitation and piRNA Sequencing Isolation and Processing of Bovine Oocytes for Small RNA Sequencing 3D Imaging and In Situ Hybridization for Uncovering the Functions of MicroRNA in Rice Anther Methods to Study Roles of piRNAs in Classic Model Organisms Cloning, Sequencing, and Linkage Analysis of piRNAs Drosophila Genetic Resources for Elucidating piRNA Pathway Generation of Stable Drosophila Ovarian Somatic Cell Lines Using the piggyBac System Methods to Study Nuclear Regulation by Other Non-coding RNAs Whole-Mount RNA FISH Combined with Immunofluorescence for the Analysis of the Telomeric Ribonucleoproteins in the Drosophila Germline CRISPR-Mediated Activation of Transposable Elements in Embryonic Stem Cells Method for Evaluating Effects of Non-coding RNAs on Nucleosome Stability Revisiting the Glass Treatment for Single-Molecule Analysis of ncRNA Function Low Input Genome-Wide DNA Methylation Analysis with Minimal Library Amplification Solid-Support Directional (SSD) RNA-Seq as a Companion Method to CLIP-Seq UPA-Seq-Based Search Method for Functional lncRNA Candidates Large-Scale Analysis of RNA–Protein Interactions for Functional RNA Motif Discovery Using FOREST Bioinformatic and Biophysical Methods to Study Non-coding RNAs Computational Methods for the Discovery and Annotation of Viral Integrations Bioinformatics Approaches for Determining the Functional Impact of Repetitive Elements on Non-coding RNAs Extending and Running the Mosquito Small RNA Genomics Resource Pipeline Preparation of Non-overlapping Transposable Elements (TEs) Annotation by Interval Tree Statistical Thermodynamics Approach for Intracellular Phase Separation

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parrish n pirna methods protocols 2022

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