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axelrod d total internal reflection fluorescence evanescence microscopies 2022

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Textbook in PDF format This book is intended as a ‘one-stop shopping’ exposition of the theory and setup details of evanescence microscopy—in particular, total internal reflection fluores cence (TIRF) and supercritical-angle fluorescence. It also includes discussions of many of the combinations of TIRF with other techniques. Every section ‘starts from the beginning’ so it should be readable by anyone with a science background that includes some physics and math. Most sections proceed to fairly advanced details. Hopefully, this will make the book usable as a research reference as well as a trigger for new ideas. In addition to the formal math, a physically intuitive explanation is provided wherever possible. Also, the topics and explanations here will hopefully not go out of date too soon, because they focus on the fundamentals underpinning the techniques. If any important techniques of optical evanescent microscopy have not been adequately covered, I apologize, and I would very much like to hear about them and possibly include them in future updates if necessary Introduction to optical evanescence Total internal reflection theory Structure in the lower-index material Emission of fluorophores near a surface Optical configurations and setup Applications of TIRF microscopy and its combination with other fluorescence techniques

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Axelrod D. Total Internal Reflection Fluorescence..Evanescence Microscopies 2022.pdf 28.1 MB

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axelrod d total internal reflection fluorescence evanescence microscopies 2022

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